Modeling of the Effects of Threonine, Valine, Isoleucine and Pyridoxal 5'-monophosphate on Biosynthetic Threonine Dehydratase Reaction

Motivation: Development of mathematical models that adequately describe moleculargenetic mechanisms is one of the main tasks in modern bioinformatics. Not only should a model be sensitive to the particulars of enzyme reactions underlying cell life activity, but also to regulation exerted on enzyme activity by metabolites and specialized cell proteins. Results: The gene network for branched-chain amino acid biosynthesis in E. coli has been reconstructed using the GeneNet system. On the basis of available information on this gene network, elementary mathematical models for enzyme reactions have been developed using generalized Hill functions. We herein give a detailed description of the mathematical model for the biosynthetic threonine dehydratase-catalyzed reaction that yields OBUT; the model includes enzyme activity regulation. Availability: The model is available on request. Gene network is available at http://wwwmgs.bionet.nsc.ru/mgs/gnw/genenet/viewer/index.shtml.